Nostochopcerol, a new antibacterial monoacylglycerol from the edible cyanobacterium Nostochopsis lobatus

A new antibacterial 3-monoacyl-sn-glycerol, nostochopcerol (1), was isolated from a cultured algal mass of the edible cyanobacterium Nostochopsis lobatus MAC0804NAN. The structure of compound 1 was established by the analysis of NMR and MS data while its chirality was established by comparison of optical rotation values with synthetically prepared authentics. Compound 1 inhibited the growth of Bacillus subtilis and Staphylococcus aureus at MIC of 50 μg/mL and 100 μg/mL, respectively.


Introduction
Cyanobacteria are widely accepted as a prolific source of unique bioactive metabolites [1]. Some cyanobacterial species are consumed as food, nutritional supplements, or folk medicines in many parts of the world [2,3], and have offered attractive opportunities for drug discovery. Results from the limited number of attempts include an antifungal lipopeptide nostofungicidine [4] and an antioxidant nostocionone [5] from Nostoc commune, an unusual antibacterial n−1 fatty acid from N. verrucosum [2], and the sacrolides, antimicrobial oxylipin macrolactones from Aphanothece sacrum [6,7].
Nostochopsis lobatus is a freshwater species distributed in every climate zone but polar regions [8]. It grows on riverbed rocks or cobbles in shallow streams and forms spherical to irregularly lobed, hollow, gelatinous colonies, with sizes reaching up to 5.5 cm in diameter [9]. Although cosmopolitic, its occurrence is dominated in tropical regions, thus food consumption of this alga is only reported from India [10] and Thailand [11]. In India, local tribes utilize it as a dietary supplement [10]. In northern Thailand, this alga occurs in dry season from November to April and is called Lon, Kai Hin (stone egg), or Dok Hin (stone flower) [11]. It is consumed as an ingredient of salad and as a folk medicine to treat pain from stomach ulcers or fever [9]. In fact, an ethanolic extract of the air-dried alga was found to inhibit the development of gastric ulcers, suppress ethyl phenylpropiolate-induced edema on ear, and decrease writhing response induced by intraperitoneal injection of acetic acid in rodent models [11], thus supporting the ethnophamacological testimonies. Moreover, radical scavenging activity [11,12], hyaluronidase inhibitory activity [13], and tyrosinase inhibitory activity [14] were detected by in vitro testings, which further raised the expectation of its richness as the source of bioactive metabolites. However, at present, only a single drug discovery attempt has been made on this alga [13], which prompted further chemical study.
We evaluated the antimicrobial activity of the ethanolic extract of this alga and found that a mid-polar fraction inhibited the growth of two Gram-positive bacteria, Bacillus subtilis and Staphylococcus aureus. Activity-guided fractionation led to the discovery of a new monoacylglycerol, nostochopcerol (1, Figure 1). Part of this study have been described in a patent [15].

Results and Discussion
A water-thawed algal mass of strain MAC0804NAN (374.6 g) was repeatedly extracted with EtOH. The combined extract was partitioned between 60% aqueous MeOH and CH 2 Cl 2 , and the latter lipophilic layer was further partitioned between 90% aqueous MeOH and n-hexane. The resulting three layers were tested against four Gram-positive bacteria, five Gram-negative bacteria, six fungi, and two yeasts, which detected antibacterial activity against two Gram-positive bacteria, Bacillus subtilis and Staphylococcus aureus, from the 90% aqueous MeOH layer. The responsible constituent, though prone to diffuse during chromatography, was purified with the guidance of antibacterial activity on ODS and Sephadex LH-20 and by reversed-phase HPLC on ODS and styrene-divinylbenzene copolymer to yield 0.7 mg of compound 1 from 113.3 mg of the solvent partition fraction. The reason for the low yield of compound 1 was eventually understood after it was determined to be a monoacylglycerol, which has a surface-active property and should have deteriorated the separation capacity of the chromatographic resins.
The molecular formula of compound 1 was established to be C 19  were observed, implying that compound 1 is a derivative of a fatty acid. Indeed, all oxygenated protons constituted a spin system (CH 2 1'-CH2'-CH 2 3') in the COSY spectrum ( Figure 1), and considering the lack of any terminal group besides CH 3 16, monoacylglycerol was the only possible structure for compound 1. This assignment was eventually proven after interpretation of the whole set of 1D and 2D NMR data. A carboxy carbon, four sp 2 methines, one oxymethine, two oxymethylenes, ten aliphatic methylenes, and a methyl group were collected from the analysis of 13 C NMR and HSQC spectra and these structural pieces were assembled into four spin systems by the COSY correlations: an ethyl group (C16-C15), a C 8 internal hydrocarbon chain with two degrees of unsaturation (C12-C11=C10-C9-C8=C7-C6-C5), three consecutive methylenes (C4-C3-C2) with a carboxy-termination, and a glyceryl moiety ( Figure 1). The Z-geometry was deduced for both double bonds (Δ 7 and Δ 10 ) from shielded chemical shift values of the allylic carbons (C6: 27.9 ppm and C12: 28.0 ppm) [16]. The first two COSY fragments were connected via the intervention of two methylene groups (CH 2  The absolute configuration of the sole chiral center at C2' in the glyceryl group was addressed by comparing the optical rotation value of compound 1 with those of synthetically prepared authentic chiral monoacylglycerols. Because (7Z,10Z)-hexadecadienoic acid was not commercially available, methyl linoleate, having the same degree of unsaturation with a longer chain length by two carbons, was used as a source of the acyl chain. Linoleic acid, obtained by saponification of methyl linoleate, was condensed either with (R)-or (S)-solketal (isopropylidene glycerol) by Steglich esterification. The resulting ester 2a or 2b was purified by reversed-phase HPLC and deprotected by a short treatment with 80% aqueous acetic acid at 58-59 °C to give 1-linoleoyl-sn-glycerol (3a) or 3-linoleoyl-snglycerol (3b), respectively (Scheme 1). Similarly, to our experience during the isolation of compound 1, swapping the order of purification and deprotection severely decreased the yields (data not shown). Compound 1 is the first non-glycosylated glycerolipid isolated from cyanobacteria [17][18][19][20]. Natural 3-acylated-sn-glycerols were also reported from the fungus Sclerotinia fructicola [21] and a brown alga Ishige sinicola [22]. The (7Z,10Z)-hexadecadienoyl group has been found in galactoglycerolipids from Chlorella [23][24][25], kale (Brassica oleracea) [26], Daphnia [27], Scheme 1: Synthesis of 1-linoleoyl-sn-glycerol (3a) and 3-linoleoyl-snglycerol (3b). and meadow buttercup (Ranunculus acris, family Ranunculaceae) [28], and as a sucrose ester from rough horsetail (Equisetum hiemale, phylum Pteridophyta) [29].

Experimental General methods
Cosmosil 75C18-PREP (Nacalai Tesque Inc., 75 µm) was used for ODS flash chromatography. NMR spectra were obtained on a Bruker AVANCE II 500 spectrometer using residual solvent peaks at δ H /δ C 3.30/49.0 ppm in CD 3 OH and 7.27/77.0 ppm in CDCl 3 as chemical shift reference signals. HR-ESITOFMS analysis was conducted on a Bruker micrOTOF mass spectrometer. Optical rotation and UV spectra were recorded on a JASCO P-1030 polarimeter and a Shimadzu UV-1800 spectrophotometer, respectively.

Extraction and isolation
A water-thawed specimen (374.6 g) was homogenized with an equal amount of Celite in EtOH (400 mL). The resulting slurry was paper-filtered to afford an ethanolic extract and an algal cake, and the latter was extracted three more times. The combined extract was concentrated in vacuo and the resulting suspension was diluted with MeOH to adjust its concentration to 60% (v/v). This was extracted with CH 2 Cl 2 for three times, and the CH 2 Cl 2 -soluble layer was partitioned between aqueous 90% MeOH and n-hexane.

Paper disk-agar diffusion method
According to a procedure described in [6], the antimicrobial potency of chromatographic fractions was evaluated by a paper disk-agar diffusion method. Fractions at each purification stage were diluted to the same concentration with MeOH, and 10 μL aliquots were impregnated into 6 mm-diameter paper disks, which were left standing until completely dried. A loop of the test organism, suspended in a small amount of water, was mixed with liquefied agar medium precooled to nearly body temperature, and the inoculated medium was quickly poured into a sterile plastic dish. The composition of the medium is 0.5% yeast extract, 1.0% tryptone, 1.0% NaCl, 0.5% glucose, and 1.5% agar. After the agar solidified, the drug-impregnated disks were placed on the medium, and the test cultures were incubated at 32 °C for a day or two until the diameters of inhibitory haloes turned measurable.

Microculture antimicrobial testing
To each well of a sterile 96-well microtiter plate was dispensed 100 μL of tryptic soy broth. Additionally, 98 μL of the same medium and 2 μL of the solutions of test compounds in MeOH or a reference antibiotic, kanamycin monosulfate, in H 2 O, were added to the wells at the top row. To make two-fold serial dilutions along the column, 100 μL aliquots from the wells of the top row were taken and added to the well in the second row and mixed gently with the pre-dispensed medium by pipetting. In the same manner, 100 μL aliquots were transferred from the second row to the third row. This operation was repeated until the transfer of diluted drug solutions reached the bottom row. The excess 100 μL in the bottom row was discarded to equalize the volume of the medium in the wells. The test strains, S. aureus FDA209P JC-1 and B. subtilis ATCC6633, were recovered on tryptic soy agar, and a loopful of bacterial masses was transferred to tryptic soy broth in a 16 mm tube. The tubes were shake-cultured for several hours at 37 °C at 306 rpm until the turbidity measured by the absorbance at 600 nm (ABS 600 ) exceeded 0.1. The liquid culture was diluted to adjust the turbidity to ABS 600 0.09-0.1 (0.5 McFarland), which corresponds to a cell density of 1.5 × 10 8 cfu/mL. This was further diluted by 75 times to prepare a cell suspension of 2.0 × 10 6 cfu/mL, of which 100 μL were dispensed to the wells to give microcultures with the final cell density of 1.0 × 10 6 cfu/mL. The plates were incubated at 37 °C for 48 h and the concentration at which the growth of microbes was completely inhibited was defined as the minimum inhibitory concentration (MIC).

Supporting Information File 1
Experimental details, characterization data and copies of spectra.